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Title: Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
Authors: Filippo Cipriani
Dominik Bernhagen
Garcia Arévalo, Carmen
González de Torre, Israel
Timmerman, Peter
Rodriguez Cabello, Jose Carlos
Issue Date: 2019
Publisher: IOP Publishing
Description: Producción Científica
Citation: Biomedical Materials, 2019 (in press)
Abstract: Biomaterial design in tissue engineering aims to identify appropriate cellular microenvironments in which cells can grow and guide new tissue formation. Despite the large diversity of synthetic polymers available for regenerative medicine, most of them fail to fully match the functional properties of their native counterparts. In contrast, the few biological alternatives employed as biomaterials lack the versatility that chemical synthesis can offer. Herein, we studied the HUVEC adhesion and proliferation properties of elastin-like recombinamers (ELRs) that were covalently functionalized with each three high-affinity and selectivity αvβ3- and α5β1-binding bicyclic RGD peptides. Next to the bicycles, ELRs were also functionalized with various integrin-binding benchmark peptides, i.e. knottin-RGD, cyclo-[KRGDf] and GRGDS, allowing for better classification of the obtained results. Covalent functionalization with the RGD peptides, as validated by MALDI-TOF analysis, guarantees flexibility and minimal steric hindrance for interactions with cellular integrins. In addition to the covalently modified RGD-ELRs, we also synthesized another benchmark ELR comprising RGD as part of the backbone. HUVEC adhesion and proliferation analysis using the PicoGreen® assay revealed a higher short-term adhesion and proliferative capacity of cells on ELR surfaces functionalized with high affinity, integrin-binding bicyclic RGD-peptides compared with the ELRs containing RGD in the backbone.
Keywords: Medicina regenerativa
Regenerative medicine
ISSN: 1748-6041
Peer Review: SI
Sponsor: European Commission (NMP-2014-646075, MSCA-ITN-2014-ETN-642687)
Ministerio de Economía, Industria y Competitividad (Projects PCIN-2015-010, 26 MAT2015-68901-R, MAT2016-78903-R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA015U16)
Publisher Version:
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:BIOFORGE - Artículos de revista

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