Skip navigation
Please use this identifier to cite or link to this item:
Title: Modulation of Ca2+ release and Ca2+ oscillations in HeLa cells and fibroblasts by mitochondrial Ca2+ uniporter stimulation
Authors: Vay, Laura
Hernández-SanMiguel, Esther
Santo Domingo, Jaime
Domínguez Lobatón, María Carmen
Moreno, Alfredo
Montero, Mayte
Álvarez Martín, Javier
Issue Date: 2007
Publisher: Wiley
Description: Producción Científica
Citation: Journal of Physiology, 2007, vol. 580, n. 1, p. 39-49
Abstract: The recent availability of activators of the mitochondrial Ca2+ uniporter allows direct testing of the influence of mitochondrial Ca2+ uptake on the overall Ca2+ homeostasis of the cell. We show here that activation of mitochondrial Ca2+ uptake by 4,4 ,4 -(4- propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT) or kaempferol stimulates histamine-induced Ca2+ release from the endoplasmic reticulum (ER) and that this effect is enhanced if the mitochondrial Na+–Ca2+ exchanger is simultaneously inhibited with CGP37157. This suggests that both Ca2+ uptake and release from mitochondria control the ability of local Ca2+ microdomains to produce feedback inhibition of inositol 1,4,5-trisphosphate receptors (InsP3Rs). In addition, the abilityof mitochondria tocontrolCa2+ release fromtheERallowsthemtomodulate cytosolic Ca2+ oscillations. In histamine stimulatedHeLa cells and human fibroblasts, both PPT and kaempferol initially stimulated and later inhibited oscillations, although kaempferol usually induced a more prolonged period of stimulation. Both compounds were also able to induce the generation of Ca2+ oscillations in previously silent fibroblasts. Our data suggest that cytosolic Ca2+ oscillations are exquisitely sensitive to the rates of mitochondrial Ca2+ uptake and release, which precisely control the size of the local Ca2+ microdomains around InsP3Rs and thus the ability to produce feedback activation or inhibition of Ca2+ release.
Keywords: Calcio en el organismo
ISSN: 0022-3751
Peer Review: SI
DOI: 10.1113/jphysiol.2006.126391
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:DEP05 - Artículos de revista

Files in This Item:
File Description SizeFormat 
ALVAREZ 21- Modulation of Ca2+ release.pdfPD-112987,71 kBAdobe PDFThumbnail

This item is licensed under a Creative Commons License Creative Commons

University of Valladolid
Powered by MIT's. DSpace software, Version 5.5