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dc.contributor.authorSayin, Esen
dc.contributor.authorHama Rashid, Rosti
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.contributor.authorElsheikh Erkan, Ahmed
dc.contributor.authorTürker Baran, Erkan
dc.contributor.authorHasirci, Vasif
dc.date.accessioned2017-07-14T11:12:16Z
dc.date.available2017-07-14T11:12:16Z
dc.date.issued2017
dc.identifier.citationBioactive Materials, 2017, Volume 2, Issue 2, Pages 71-81es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/24424
dc.descriptionProducción Científicaes
dc.description.abstractThe ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen – silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films. The Young's modulus and the ultimate tensile strength (UTS) of untreated films were 0.58 ± 0.13 MPa and 0.18 ± 0.05 MPa, respectively. After 28 days of cell culture, ADSC seeded film had a Young's modulus of 1.21 ± 0.42 MPa and UTS of 0.32 ± 0.15 MPa which were about 3 fold higher than HOB seeded films. The difference in Young's modulus was statistically significant (p: 0.02). ADSCs attached, proliferated and mineralized better than the HOBs. In the light of these results, ADSCs served as a better cell source than HOBs for bone tissue engineering of collagen-fibroin-ELR based constructs used in this study. We have thus shown the enhancement in the tensile mechanical properties of the bone tissue engineered scaffolds by using ADSCs.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherRoyal Society of Chemistryes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationCélulas madrees
dc.subject.classificationHuesoses
dc.titleHuman adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blendes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doihttps://doi.org/10.1016/j.bioactmat.2017.04.001es
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S2452199X17300038es
dc.peerreviewedSIes
dc.description.projectMinisterio de Economía, Industria y Competitividad (Project MAT2013-42473-R and MAT2015-68901R)es
dc.description.projectJunta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. (VA244U13, VA313U14 and VA015U16)es
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/642687
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/646075
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/278557
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317306
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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