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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/24424

    Título
    Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend
    Autor
    Sayin, Esen
    Hama Rashid, Rosti
    Rodríguez Cabello, José CarlosAutoridad UVA Orcid
    Elsheikh Erkan, Ahmed
    Türker Baran, Erkan
    Hasirci, Vasif
    Año del Documento
    2017
    Editorial
    Royal Society of Chemistry
    Descripción
    Producción Científica
    Documento Fuente
    Bioactive Materials, 2017, Volume 2, Issue 2, Pages 71-81
    Abstract
    The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen – silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films. The Young's modulus and the ultimate tensile strength (UTS) of untreated films were 0.58 ± 0.13 MPa and 0.18 ± 0.05 MPa, respectively. After 28 days of cell culture, ADSC seeded film had a Young's modulus of 1.21 ± 0.42 MPa and UTS of 0.32 ± 0.15 MPa which were about 3 fold higher than HOB seeded films. The difference in Young's modulus was statistically significant (p: 0.02). ADSCs attached, proliferated and mineralized better than the HOBs. In the light of these results, ADSCs served as a better cell source than HOBs for bone tissue engineering of collagen-fibroin-ELR based constructs used in this study. We have thus shown the enhancement in the tensile mechanical properties of the bone tissue engineered scaffolds by using ADSCs.
    Palabras Clave
    Células madre
    Huesos
    Revisión por pares
    SI
    DOI
    10.1016/j.bioactmat.2017.04.001
    Patrocinador
    Ministerio de Economía, Industria y Competitividad (Project MAT2013-42473-R and MAT2015-68901R)
    Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. (VA244U13, VA313U14 and VA015U16)
    Patrocinador
    info:eu-repo/grantAgreement/EC/H2020/642687
    info:eu-repo/grantAgreement/EC/H2020/646075
    info:eu-repo/grantAgreement/EC/FP7/278557
    info:eu-repo/grantAgreement/EC/FP7/317306
    Version del Editor
    http://www.sciencedirect.com/science/article/pii/S2452199X17300038
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/24424
    Derechos
    openAccess
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    • BIOFORGE - Artículos de revista [89]
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    Attribution-NonCommercial-NoDerivatives 4.0 InternationalLa licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 International

    Universidad de Valladolid

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