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Título
A double safety lock tumor-specific device for suicide gene therapy in breast cancer
Autor
Año del Documento
2020
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Cancer Letters, 2020, vol. 470. p. 43-53
Abstract
The complexity and continuous evolution of cancer make the design of novel strategies of treatment a constant challenge in biomedicine. Moreover, most of cancer treatments are still not tumor-specific and provoke high systemic toxicity. Herein we have developed a novel selective nanodevice to eliminate tumor cells while leaving healthy ones intact. To achieve this objective, a polyplex carrier, comprising an elastin like-recombinamer covalently conjugated to an aptamer and complexed with therapeutic DNA, was tested. This carrier forms a double-lock multifunctional device due to specific binding to a tumor cell marker and the selective expression of therapeutic DNA inside human breast-cancer cells. Due to the stability provided by ELRs, the homogeneous population of polyplexes obtained showed selective toxicity against cancer cells in in vitro and in vivo assay. Inhibition of tumor progression was detected early being very significant at the end point, with a dose-dependent reduction in tumor mass. Histological studies revealed a specific reduction in tumor parenchyma and in specific tumor cell markers. These results represent an important step toward the rational development of an efficient, safe and more specialized gene-delivery device for tumor therapy.
Palabras Clave
Elastin-like recombinamers
Recombinantes tipo elastina
Nanocarriers
Nanoportadores
Suicide gene therapy
Terapia génica suicida
Breast cancer
Cáncer de mama
ISSN
0304-3835
Revisión por pares
SI
Patrocinador
Fondo Social Europeo - FEDER- Unión Europea (project MP-2014-646075
Ministerio de Economía, Industria y Competitividad (project PCIN-2015-010 / MAT2015-68901-R / MAT2016-78903-R)
Junta de Castilla y León (project VA317P18)
Ministerio de Economía, Industria y Competitividad (project PCIN-2015-010 / MAT2015-68901-R / MAT2016-78903-R)
Junta de Castilla y León (project VA317P18)
Version del Editor
Propietario de los Derechos
© 2020 Elsevier
Idioma
eng
Tipo de versión
info:eu-repo/semantics/draft
Derechos
openAccess
Collections
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