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dc.contributor.authorCidad Velasco, María del Pilar
dc.contributor.authorMoreno Domínguez, Alejandro
dc.contributor.authorNovensá, Laura
dc.contributor.authorRoqué, Mercé
dc.contributor.authorBarquín, Leire
dc.contributor.authorHeras i Fortuny, Maria Magdalena
dc.contributor.authorPérez García, María Teresa 
dc.contributor.authorLópez López, José Ramón 
dc.date.accessioned2020-12-22T10:25:28Z
dc.date.available2020-12-22T10:25:28Z
dc.date.issued2010
dc.identifier.citationArteriosclerosis, Thrombosis, and Vascular Biology, 2010, vol. 30, n. 6. p. 1203-1211es
dc.identifier.issn1524-4636es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/44599
dc.descriptionProducción Científicaes
dc.description.abstractObjective: Vascular smooth muscle cells (VSMCs) contribute significantly to occlusive vascular diseases by virtue of their ability to switch to a noncontractile, migratory, and proliferating phenotype. Although the participation of ion channels in this phenotypic modulation (PM) has been described previously, changes in their expression are poorly defined because of their large molecular diversity. We obtained a global portrait of ion channel expression in contractile versus proliferating mouse femoral artery VSMCs, and explored the functional contribution to the PM of the most relevant changes that we observed. Methods and Results: High-throughput real-time polymerase chain reaction of 87 ion channel genes was performed in 2 experimental paradigms: an in vivo model of endoluminal lesion and an in vitro model of cultured VSMCs obtained from explants. mRNA expression changes showed a good correlation between the 2 proliferative models, with only 2 genes, Kv1.3 and Kvβ2, increasing their expression on proliferation. The functional characterization demonstrates that Kv1.3 currents increased in proliferating VSMC and that their selective blockade inhibits migration and proliferation. Conclusion: These findings establish the involvement of Kv1.3 channels in the PM of VSMCs, providing a new therapeutical target for the treatment of intimal hyperplasia.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Heart Associationes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/*
dc.subject.classificationGene expressiones
dc.subject.classificationExpresión génicaes
dc.subject.classificationIon channelses
dc.subject.classificationCanales iónicoses
dc.subject.classificationRestenosises
dc.subject.classificationVascular smooth musclees
dc.subject.classificationMúsculo liso vasculares
dc.titleCharacterization of ion channels involved in the proliferative response of femoral artery smooth muscle cellses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2010 American Heart Associationes
dc.identifier.doi10.1161/ATVBAHA.110.205187es
dc.relation.publisherversionhttps://www.ahajournals.org/doi/10.1161/ATVBAHA.110.205187es
dc.peerreviewedSIes
dc.description.projectMinisterio de Sanidad, Consumo y Bienestar Social - Instituto de Salud Carlos III (grants R006/009, FS041139-0 and PI041044)es
dc.description.projectMinisterio de Ciencia, Innovación y Universidades (grants BFU2004-05551 and BFU2007-61524)es
dc.description.projectJunta de Castilla y León (grant GR242)es
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Unported*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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