Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/45075
Título
Mitochondrial [Ca2+] oscillations driven by local high [Ca2+] domains generated by spontaneous electric activity
Autor
Año del Documento
2001
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Journal of Biological Chemistry, 2001, vol. 276, n. 43. p. 40293-40297
Abstract
Mitochondria take up calcium during cell activation thus shaping Ca2+ signaling and exocytosis. In turn, Ca2+ uptake by mitochondria increases respiration and ATP synthesis. Targeted aequorins are excellent Ca2+ probes for subcellular analysis, but single-cell imaging has proven difficult. Here we combine virus-based expression of targeted aequorins with photon-counting imaging to resolve dynamics of the cytosolic, mitochondrial, and nuclear Ca2+ signals at the single-cell level in anterior pituitary cells. These cells exhibit spontaneous electric activity and cytosolic Ca2+oscillations that are responsible for basal secretion of pituitary hormones and are modulated by hypophysiotrophic factors. Aequorin reported spontaneous [Ca2+] oscillations in all the three compartments, bulk cytosol, nucleus, and mitochondria. Interestingly, a fraction of mitochondria underwent much larger [Ca2+] oscillations, which were driven by local high [Ca2+] domains generated by the spontaneous electric activity. These oscillations were large enough to stimulate respiration, providing the basis for local tune-up of mitochondrial function by the Ca2+ signal.
Palabras Clave
Mitochondria
Mitocondrias
Calcium
Calcio
Cell activation
Activación celular
ISSN
0021-9258
Revisión por pares
SI
Patrocinador
Dirección General de Enseñanza Superior (grants PB97-0474, APC1999-011 and 1FD97-1725-C02-02)
Version del Editor
Propietario de los Derechos
© 2001 Elsevier
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Collections
Files in this item
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Unported