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dc.contributor.authorBueno, Juan M.
dc.contributor.authorÁvila, Francisco J.
dc.contributor.authorLorenzo Martín, Elvira
dc.contributor.authorGallego Muñoz, Patricia 
dc.contributor.authorMartínez García, María del Carmen 
dc.date.accessioned2021-02-09T10:02:20Z
dc.date.available2021-02-09T10:02:20Z
dc.date.issued2021
dc.identifier.citationBiomedical Optics Express Vol. 12, Issue 2, pp. 756-765 (2021)es
dc.identifier.issn2156-7085es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/45227
dc.descriptionProducción Científicaes
dc.description.abstractThe organization of the corneal stoma is modified due to different factors, including pathology, surgery or external damage. Here the changes in the organization of the corneal collagen fibers during natural healing after chemical burn are investigated using second harmonic generation (SHG) imaging. Moreover, the structure tensor (ST) was used as an objective tool for morphological analyses at different time points after burn (up to 6 months). Unlike control corneas that showed a regular distribution, the collagen pattern at 1 month of burn presented a non-organized arrangement. SHG signal levels noticeably decreased and individual fibers were hardly visible. Over time, the healing process led to a progressive re-organization of the fibers that could be quantified through the ST. At 6 months, the stroma distribution reached values similar to those of control eyes and a dominant direction of the fibers re-appeared. The present results show that SHG microscopy imaging combined with the ST method is able to objectively monitor the temporal regeneration of the corneal organization after chemical burn. Future implementations of this approach into clinically adapted devices would help to diagnose and quantify corneal changes, not only due to chemical damages, but also as a result of disease or surgical procedures.es
dc.description.abstractes
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherOptical Society of America (OSA)es
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCórnea - cirugíaes
dc.subject.classificationColágeno corneales
dc.subject.classificationCorneal collagenes
dc.titleAssessment of the corneal collagen organization after chemical burn using second harmonic generation microscopyes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2021 Optical Society of Americaes
dc.rights.holder
dc.identifier.doi10.1364/BOE.412819es
dc.relation.publisherversionhttps://www.osapublishing.org/boe/fulltext.cfm?uri=boe-12-2-756&id=446454#articleBodyes
dc.identifier.publicationfirstpage756es
dc.identifier.publicationissue2es
dc.identifier.publicationtitleBiomedical Optics Expresses
dc.identifier.publicationvolume12es
dc.peerreviewedSIes
dc.description.other© 2021 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
dc.description.projectMinisterio de Sanidad. Instituto de Salud Carlos III (PI15/01906)es
dc.description.projectFundación Séneca (19897/GERM/15)es
dc.identifier.essn2156-7085es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco2407 Biología Celulares


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