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Título
Mineralizing coating on 3D printed scaffolds for the promotion of osseointegration
Autor
Año del Documento
2022
Editorial
Frontiers Media
Descripción
Producción Científica
Documento Fuente
Frontiers in Bioengineering and Biotechnology, 2022, vol. 10, artículo 836386
Zusammenfassung
Design and fabrication of implants that can perform better than autologous bone grafts
remain an unmet challenge for the hard tissue regeneration in craniomaxillofacial
applications. Here, we report an integrated approach combining additive
manufacturing with supramolecular chemistry to develop acellular mineralizing 3D
printed scaffolds for hard tissue regeneration. Our approach relies on an elastin-like
recombinamer (ELR) coating designed to trigger and guide the growth of ordered apatite
on the surface of 3D printed nylon scaffolds. Three test samples including a) uncoated
nylon scaffolds (referred to as “Uncoated”), b) ELR coated scaffolds (referred to as “ELR
only”), and c) ELR coated and in vitro mineralized scaffolds (referred to as “Pre-
mineralized”) were prepared and tested for in vitro and in vivo performance. All test
samples supported normal human immortalized mesenchymal stem cell adhesion,
growth, and differentiation with enhanced cell proliferation observed in the “Pre-
mineralized” samples. Using a rabbit calvarial in vivo model, ‘Pre-mineralized’ scaffolds
also exhibited higher bone ingrowth into scaffold pores and cavities with higher tissue-
implant integration. However, the coated scaffolds (“ELR only” and “Pre-mineralized”) did
not exhibit significantly more new bone formation compared to “Uncoated” scaffolds.
Overall, the mineralizing coating offers an opportunity to enhance integration of 3D printed
bone implants. However, there is a need to further decipher and tune their immunologic
response to develop truly osteoinductive/conductive surfaces.
Materias (normalizadas)
Cirugía maxilofacial
Materias Unesco
2407 Biología Celular
3213.13 Ortodoncia-Estomatología
Palabras Clave
Biomineralization
Bone regeneration
Regeneración ósea
3D printing
Impresión 3D
ISSN
2296-4185
Revisión por pares
SI
Patrocinador
AO foundation (AOCMF - 17–19M)
ERC Starting Grant (STROFUNSCAFF) ERC Proof-of-concept Grant (MINGRAFT), the Engineering and Physical Sciences Research Council (EP/N006615/1)
Medical Research Council (United Kingdom Regenerative Medicine Platform Hub Acellular Smart Materials 3D Architecture, MR/R015651/1)
Spanish Government (PID2019 - 110709RB - 100, RED2018 – 102417 - T)
Junta de Castilla y León (VA317P18, Infrared 2018 - UVA06)
Interreg V España Portugal POCTEP (0624_2IQBIONEURO_6_E), Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
ERC Starting Grant (STROFUNSCAFF) ERC Proof-of-concept Grant (MINGRAFT), the Engineering and Physical Sciences Research Council (EP/N006615/1)
Medical Research Council (United Kingdom Regenerative Medicine Platform Hub Acellular Smart Materials 3D Architecture, MR/R015651/1)
Spanish Government (PID2019 - 110709RB - 100, RED2018 – 102417 - T)
Junta de Castilla y León (VA317P18, Infrared 2018 - UVA06)
Interreg V España Portugal POCTEP (0624_2IQBIONEURO_6_E), Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
Version del Editor
Propietario de los Derechos
© The author(s)
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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