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Título
Exon-Intron Structure and Evolution of the Lipocalin Gene Family
Año del Documento
2003
Editorial
Society for Molecular Biology and Evolution
Descripción
Innovación Educativa
Documento Fuente
Molecular Biology and Evolution, 2003, vol. 20, n. 5, p. 775-783
Resumen
The Lipocalins are an ancient protein family whose expression is currently confirmed in bacteria, protoctists, plants,
arthropods, and chordates. The evolution of this protein family has been assessed previously using amino acid sequence
phylogenies. In this report we use an independent set of characters derived from the gene structure (exon-intron
arrangement) to infer a new lipocalin phylogeny. We also present the novel gene structure of three insect lipocalins. The
position and phase of introns are well preserved among lipocalin clades when mapped onto a protein sequence alignment,
suggesting the homologous nature of these introns. Because of this homology, we use the intron position and phase of 23
lipocalin genes to reconstruct a phylogeny by maximum parsimony and distance methods. These phylogenies are very
similar to the phylogenies derived from protein sequence. This result is confirmed by congruence analysis, and
a consensus tree shows the commonalities between the two source trees. Interestingly, the intron arrangement phylogeny
shows that metazoan lipocalins have more introns than other eukaryotic lipocalins, and that intron gains have occurred in
the C-termini of chordate lipocalins. We also analyze the relationship of intron arrangement and protein tertiary structure,
as well as the relationship of lipocalins with members of the proposed structural superfamily of calycins. Our congruence
analysis validates the gene structure data as a source of phylogenetic information and helps to further refine our
hypothesis on the evolutionary history of lipocalins.
Materias (normalizadas)
Lipocainas
Calciproteinas
ISSN
0737-4038
Revisión por pares
SI
Idioma
eng
Derechos
openAccess
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