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dc.contributor.authorMartín González, Diego
dc.contributor.authorBordel Velasco, Sergio 
dc.contributor.authorSolis, Selvin
dc.contributor.authorGutierrez Merino, Jorge
dc.contributor.authorSantos Beneit, Fernando
dc.date.accessioned2023-12-13T12:17:04Z
dc.date.available2023-12-13T12:17:04Z
dc.date.issued2023
dc.identifier.citationMicroorganisms, 2023, Vol. 11, Nº. 2, 456es
dc.identifier.issn2076-2607es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/63600
dc.descriptionProducción Científicaes
dc.description.abstractTwo efficient feather-degrading bacteria were isolated from honeybee samples and identified as Bacillus sonorensis and Bacillus licheniformis based on 16S rRNA and genome sequencing. The strains were able to grow on chicken feathers as the sole carbon and nitrogen sources and degraded the feathers in a few days. The highest keratinase activity was detected by the B. licheniformis CG1 strain (3800 U × mL−1), followed by B. sonorensis AB7 (1450 U × mL−1). Keratinase from B. licheniformis CG1 was shown to be active across a wide range of pH, potentially making this strain advantageous for further industrial applications. All isolates displayed antimicrobial activity against Micrococcus luteus; however, only B. licheniformis CG1 was able to inhibit the growth of Mycobacterium smegmatis. In silico analysis using BAGEL and antiSMASH identified gene clusters associated with the synthesis of non-ribosomal peptide synthetases (NRPS), polyketide synthases (PKSs) and/or ribosomally synthesized and post-translationally modified peptides (RiPPs) in most of the Bacillus isolates. B. licheniformis CG1, the only strain that inhibited the growth of the mycobacterial strain, contained sequences with 100% similarity to lichenysin (also present in the other isolates) and lichenicidin (only present in the CG1 strain). Both compounds have been described to display antimicrobial activity against distinct bacteria. In summary, in this work, we have isolated a strain (B. licheniformis CG1) with promising potential for use in different industrial applications, including animal nutrition, leather processing, detergent formulation and feather degradation.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherMDPIes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBacillus (Bacteria)es
dc.subjectHoneyes
dc.subjectMieles
dc.subjectBeeses
dc.subjectAbejases
dc.subjectKeratines
dc.subjectProteinses
dc.subjectAntibioticses
dc.subjectFeedses
dc.subjectBiodegradationes
dc.subjectBiodegradaciónes
dc.subjectMicroorganismses
dc.subjectMicroorganismoses
dc.subjectMicrobiologyes
dc.subject.classificationBacillus licheniformises
dc.titleCharacterization of Bacillus strains from natural honeybee products with high keratinolytic activity and antimicrobial potentiales
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2023 The authorses
dc.identifier.doi10.3390/microorganisms11020456es
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/11/2/456es
dc.identifier.publicationfirstpage456es
dc.identifier.publicationissue2es
dc.identifier.publicationtitleMicroorganismses
dc.identifier.publicationvolume11es
dc.peerreviewedSIes
dc.description.projectFondo Europeo de Desarrollo Regional (FEDER) - (TCUE 2021–2023) - (project 067/229111)es
dc.identifier.essn2076-2607es
dc.rightsAtribución 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco3108.01 Bacteriases
dc.subject.unesco2302.27 Proteínases
dc.subject.unesco2414.01 Antibióticoses
dc.subject.unesco2414 Microbiologíaes


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