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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/65786

    Título
    Multi-omics analysis revealed increased de novo synthesis of serine and lower activity of the methionine cycle in breast cancer cell lines
    Autor
    Pankevičiūtė Bukauskienė, Monika
    Mikalayeva, Valeryia
    Žvikas, Vaidotas
    Skeberdis, Vytenis Arvydas
    Bordel Velasco, SergioAutoridad UVA Orcid
    Año del Documento
    2023
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    Molecules, 2023, Vol. 28, Nº. 11, 4535
    Zusammenfassung
    A pipeline for metabolomics, based on UPLC-ESI-MS, was tested on two malignant breast cancer cell lines of the sub-types ER(+), PR(+), and HER2(3+) (MCF-7 and BCC), and one non-malignant epithelial cancer cell line (MCF-10A). This allowed us to quantify 33 internal metabolites, 10 of which showed a concentration profile associated with malignancy. Whole-transcriptome RNA-seq was also carried out for the three mentioned cell lines. An integrated analysis of metabolomics and transcriptomics was carried out using a genome-scale metabolic model. Metabolomics revealed the depletion of several metabolites that have homocysteine as a precursor, which was consistent with the lower activity of the methionine cycle caused by lower expression of the AHCY gene in cancer cell lines. Increased intracellular serine pools in cancer cell lines appeared to result from the over-expression of PHGDH and PSPH, which are involved in intracellular serine biosynthesis. An increased concentration of pyroglutamic acid in malignant cells was linked to the overexpression of the gene CHAC1.
    Materias (normalizadas)
    Breast - Cancer
    Mamas - Cáncer
    Cancer research
    Pharmacology
    Toxicology
    Drugs - Therapeutic use
    Medicamentos
    Metabolomics
    Metabolism - Research
    Medical genetics
    Genética médica
    Materias Unesco
    3209 Farmacología
    3214 Toxicología
    32 Ciencias Médicas
    ISSN
    1420-3049
    Revisión por pares
    SI
    DOI
    10.3390/molecules28114535
    Patrocinador
    Consejo de Investigación de Lituania - (grant S-SEN-20-6)
    Version del Editor
    https://www.mdpi.com/1420-3049/28/11/4535
    Propietario de los Derechos
    © 2023 The authors
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/65786
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP48 - Artículos de revista [265]
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    Dateien zu dieser Ressource
    Nombre:
    Multi-Omics-Analysis.pdf
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    Universidad de Valladolid

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