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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/67727

    Título
    Predominantly pro-inflammatory phenotype with mixed M1/M2 polarization of peripheral blood classical monocytes and monocyte-derived macrophages among patients with excessive ethanol intake
    Autor
    Fernández Regueras, María
    Carbonell, Cristina
    Salete Granado, Daniel
    García, Juan Luis
    Gragera, Marcos
    Pérez Nieto, María Ángeles
    Morán Plata, Francisco Javier
    Mayado, Andrea
    Torres, Jorge Luis
    Corchete Sánchez, Luis Antonio
    Usategui Martín, RicardoAutoridad UVA Orcid
    Bueno Martínez, ElenaAutoridad UVA Orcid
    Rojas Pirela, Maura
    Sabio, Guadalupe
    González Sarmiento, Rogelio
    Orfao, Alberto
    Laso, Francisco Javier
    Almeida, Julia
    Marcos, Miguel
    Año del Documento
    2023
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    Antioxidants, 2023, Vol. 12, Nº. 9, 1708
    Abstract
    Excessive alcohol consumption impairs the immune system, induces oxidative stress, and triggers the activation of peripheral blood (PB) monocytes, thereby contributing to alcoholic liver disease (ALD). We analyzed the M1/M2 phenotypes of circulating classical monocytes and macrophage-derived monocytes (MDMs) in excessive alcohol drinkers (EADs). PB samples from 20 EADs and 22 healthy controls were collected for isolation of CD14+ monocytes and short-term culture with LPS/IFNγ, IL4/IL13, or without stimulation. These conditions were also used to polarize MDMs into M1, M2, or M0 phenotypes. Cytokine production was assessed in the blood and culture supernatants. M1/M2-related markers were analyzed using mRNA expression and surface marker detection. Additionally, the miRNA profile of CD14+ monocytes was analyzed. PB samples from EADs exhibited increased levels of pro-inflammatory cytokines. Following short-term culture, unstimulated blood samples from EADs showed higher levels of soluble TNF-α and IL-8, whereas monocytes expressed increased levels of surface TNF-α and elevated mRNA expression of pro-inflammatory cytokines and inducible nitric oxide synthase. MDMs from EADs showed higher levels of TNF-α and CD206 surface markers and increased IL-10 production. LPS/IFNγ induced higher mRNA expression of Nrf2 only in the controls. miRNA analysis revealed a distinctive miRNA profile that is potentially associated with liver carcinogenesis and ALD through inflammation and oxidative stress. This study confirms the predominantly pro-inflammatory profile of PB monocytes among EADs and suggests immune exhaustion features in MDMs.
    Materias (normalizadas)
    Alcohol Use Disorders
    Excessive alcohol drinkers
    Monocyte/macrophage phenotype
    M1
    M2
    Innate immunity
    Flow cytometry
    MicroRNA
    Materias Unesco
    6113.01 Alcoholismo
    2412 Inmunología
    2415 Biología Molecular
    2407 Biología Celular
    ISSN
    2076-3921
    Revisión por pares
    SI
    DOI
    10.3390/antiox12091708
    Patrocinador
    Instituto de Salud Carlos III y Unión Europea - ( projects PI10/01692, RD16/0017/0023, PI20/00743, and INT21/00065)
    Junta de Castilla y León - (projects GRS 531/A/10, GRS 859/A/13, GRS 2388/A/21, and GRS 2648/A/22)
    Instituto de Investigaciones Biomédicas de Salamanca (IBSAL) y la Sociedad Hispano-Leonés-Cántabra de Medicina Interna (SOCALMI) 2022- (grant IBI19/00013)
    Instituto de Salud Carlos III y Unión Europea - (project FI21/00189)
    Instituto de Salud Carlos III y Unión Europea (NextGeneration UE) - (project CM22/00033)
    Version del Editor
    https://www.mdpi.com/2076-3921/12/9/1708
    Propietario de los Derechos
    © 2023 The authors
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/67727
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP05 - Artículos de revista [198]
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