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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/71461

    Título
    Ex-vivo method to quantifiably evaluate the staining effectiveness of anterior lens capsule dyes
    Autor
    Fernández Bueno, IvánAutoridad UVA
    Usategui Martín, RicardoAutoridad UVA Orcid
    Pastor Jimeno, José CarlosAutoridad UVA
    Andrés Iglesias, CristinaAutoridad UVA Orcid
    Año del Documento
    2021
    Descripción
    Producción Científica
    Documento Fuente
    Translational Vision Science & Technology 2021;10:17
    Resumo
    Purpose: High frequency of cataracts and the requirements of new European Union regulations for medical devices require the availability of preclinical models to adequatelyevaluateanteriorlens capsuledyes beforetheir usein patients.Herein, we describeanexvivomethodtoquantifiablyevaluatethemacroscopicandmicroscopic stainingeffectivenessofanteriorlenscapsuledyesusingporcineeyes. Methods: Commercially available trypan blue–based products or physiological saline solution(negativecontrol)wasinjectedintoporcineeyes.Anteriorpoleandlens(after extraction) were macroscopically photographed, and the images were quantitatively analyzed. Lenses were histologically processed, and the staining intensity microscopi- callywassemiquantified. Results: Macroscopic evaluation of the anterior pole revealed bluish staining of the anteriorcapsule;however,thiscoloringcannotbemacroscopicallydiscernedafterlens extraction.Quantitativeimageanalysesshowedsignificant(P < 0.01)stainingofthelens capsule compared to the negative control, but not significant (P > 0.05) between the productstested.Quantitativeanalysisofdyingonlensimagescouldnotbeperformed. Microscopicsemiquantificationofthecapsulestainingintensityallowsustoappreciate differencesbetweenproducts. Conclusions: The described method is a quick and useful tool for macroscopic evalu- ation by surgeons to choose an anterior capsule staining for use during everyday surgeries, and a more specific microscopic evaluation also allows us to determine the effectivenessandusefulnessoftheseproducts. Translational Relevance: This method satisfies preclinical effectiveness evaluations requiredbyEuropeanUnionregulationsandcomplementsthesafetyandtoxicityevalu- ationsthatnewproductsmustguaranteebeforetheyenterthemarketandareusedin clinicalpractice.
    ISSN
    2164-2591
    Revisión por pares
    SI
    DOI
    10.1167/tvst.10.14.17
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/71461
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
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    • DEP11 - Artículos de revista [242]
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    Universidad de Valladolid

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