Gender Dimorphism in Skeletal Muscle Leptin Receptors, Serum Leptin and Insulin Sensitivity

Guerra, Borja; Fuentes, Teresa; Delgado-Guerra, Safira; Guadalupe-Grau, Amelia; Olmedillas, Hugo; Santana, Alfredo; Ponce-Gonzalez, Jesus Gustavo; Dorado, Cecilia; Calbet, José A. L.

doi:10.1371/journal.pone.0003466

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Título

Gender Dimorphism in Skeletal Muscle Leptin Receptors, Serum Leptin and Insulin Sensitivity

Autor

Guerra, Borja

Fuentes, Teresa

Delgado-Guerra, Safira

Guadalupe-Grau, Amelia

Olmedillas, Hugo

Santana, Alfredo

Ponce-Gonzalez, Jesus Gustavo

Dorado, Cecilia

Calbet, José A. L.

Año del Documento

2008-10

Editorial

PLoS ONE

Descripción

Producción Científica

Documento Fuente

Guerra B, Fuentes T, Delgado-Guerra S, Guadalupe-Grau A, Olmedillas H, Santana A, Ponce-Gonzalez JG, Dorado C, Calbet JA. Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity. PLoS One. 2008;3(10):e3466. doi: 10.1371/journal.pone.0003466. Epub 2008 Oct 21. PMID: 18941624; PMCID: PMC2565105.

Resumen

Todetermineifthereisagenderdimorphismintheexpressionof leptinreceptors(OB-R170,OB-R128andOB-R98)andthe proteinsuppressorofcytokinesignaling3(SOCS3) inhumanskeletalmuscle, theproteinexpressionofOB-R,perilipinA, SOCS3andalpha-tubulinwasassessedbyWesternblot inmusclebiopsiesobtainedfromthem.vastus lateralis inthirty fourmen(age=27.166.8yr) andthirty-threewomen(age=26.766.7yr). Basal seruminsulinconcentrationandHOMA weresimilar inbothgenders.Serumleptinconcentrationwas3.4timeshigher inwomencomparedtomen(P,0.05)and thisdifferenceremainedsignificantafteraccountingforthedifferencesinpercentageofbodyfatorsolubleleptinreceptor. OB-Rproteinwas 41%(OB-R170, P,0.05) and163%(OB-R128, P,0.05) greater inwomen thanmen. Therewas no relationshipbetweenOB-Rexpressionandtheserumconcentrationsof leptinor17b-estradiol. Inmen,muscleOB-R128 proteinwas inverselyrelatedtoserumfreetestosterone. Inwomen,OB-R98andOB-R128wereinverselyrelatedtototal serumtestosteroneconcentration,andOB-R128toserumfreetestosteroneconcentration.SOCS3proteinexpressionwas similarinmenandwomenandwasnotrelatedtoOB-R. Inwomen,therewasaninverserelationshipbetweenthelogarithm of free testosteroneandSCOS3proteincontent inskeletalmuscle (r=20.46, P,0.05). Insummary, there is agender dimorphisminskeletalmuscleleptinreceptorsexpression,whichcanbepartlyexplainedbytheinfluenceoftestosterone. SOCS3 expression in skeletal muscle is not up-regulated inwomen, despite very high serum leptin concentrations comparedtomen.Thecirculatingformof theleptinreceptorcannotbeusedasasurrogatemeasureof theamountof leptinreceptorsexpressedinskeletalmuscles.

Revisión por pares

DOI

10.1371/journal.pone.0003466