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Title: Cartilage Regeneration in Preannealed Silk Elastin-Like Co-Recombinamers Injectable Hydrogel Embedded with Mature Chondrocytes in an Ex Vivo Culture Platform
Authors: Cipriani, Filippo
Krüger, Melanie
González de Torre, Israel
Quintanilla Sierra, Luis
Alonso Rodrigo, Matilde
Kock, Linda
Rodríguez Cabello, José Carlos
Issue Date: 2018
Publisher: American Chemical Society
Description: Producción Científica
Citation: Biomacromolecules, 2018, 19 (11), pp 4333–4347
Abstract: Tissue engineering for cartilage repair requires biomaterials that show rapid gelation and adequate mechanical properties. Although the use of hydrogel is the most promising biomaterial, it often lacks in rigidity and anchorage of cells when they are surrounded by synovial fluid while they are subjected to heavy loads. We developed and produced the Silk Elastin-Like co-Recombinamer (SELR), which contains both the physical interaction from elastin motifs and from silk motifs. In the first part of this work, we set up and optimized a preannealing treatment based on the evolution of silk motifs into β-sheet structures in order to fulfill the required mechanical properties of hydrogels for cartilage repair. The new preannealed SELRs (pA(EIS)2-(I5R)6) were characterized with the combination of several experimental techniques (CD, TEM, SEM, and rheology) to provide a deep insight into the material features. Finally, the regeneration properties of the pA(EIS)2-(I5R)6 hydrogel embedded with chondrocytes were evaluated. After 4 weeks of culturing in a standardized and representative ex vivo model, the biochemical and histological analysis revealed the production of glycosaminglycans and collagen. Moreover, the immunohistochemistry showed the absence of fibro-cartilage and the presence of hyaline cartilage. Hence, we conclude that the pA(EIS)2-(I5R)6 hydrogel presents improved mechanical properties while conserving the injectability, which leads to successful regeneration of hyaline cartilage in an ex vivo model.
Classification: Regeneración del cartílago
Cartilage Regeneration
ISSN: 1525-7797
Peer Review: SI
Sponsor: Ministerio de Economía, Industria y Competitividad (Proyects PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R,MAT2016-79435-R
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. Project VA015U16)
European Union’sHorizon 2020 research and innovation programme under theMarie Sklodowska-Curie grant agreement No. 64268
Programme: info:eu-repo/grantAgreement/EC/H2020/642687
Publisher Version:
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Documentos OpenAire(Open Access Infrastructure for Research in Europe)
BIOFORGE - Artículos de revista

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