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Title: Novel haloalkaliphilic methanotrophic bacteria: An attempt for enhancing methane bio-refinery
Authors: Cantera Ruiz de Pellón, Sara
Sánchez Andrea, Irene
Sadornila, Lidia J.
García Encina, Pedro
Stamsb, Alfons J.M.
Muñoz Torre, Raúl
Issue Date: 2019
Publisher: Elsevier
Description: Producción Científica
Citation: Journal of Environmental Management, 2019, Volume 231, Pages 1091-1099
Abstract: Methane bioconversion into products with a high market value, such as ectoine or hydroxyectoine, can be optimized via isolation of more efficient novel methanotrophic bacteria. The research here presented focused on the enrichment of methanotrophic consortia able to co-produce different ectoines during CH4 metabolism. Four different enrichments (Cow3, Slu3, Cow6 and Slu6) were carried out in basal media supplemented with 3 and 6% NaCl, and using methane as the sole carbon and energy source. The highest ectoine accumulation (∼20 mg ectoine g biomass−1) was recorded in the two consortia enriched at 6% NaCl (Cow6 and Slu6). Moreover, hydroxyectoine was detected for the first time using methane as a feedstock in Cow6 and Slu6 (∼5 mg g biomass−1). The majority of the haloalkaliphilic bacteria identified by 16S rRNA community profiling in both consortia have not been previously described as methanotrophs. From these enrichments, two novel strains (representing novel species) capable of using methane as the sole carbon and energy source were isolated: Alishewanella sp. strain RM1 and Halomonas sp. strain PGE1. Halomonas sp. strain PGE1 showed higher ectoine yields (70–92 mg ectoine g biomass−1) than those previously described for other methanotrophs under continuous cultivation mode (∼37–70 mg ectoine g biomass−1). The results here obtained highlight the potential of isolating novel methanotrophs in order to boost the competitiveness of industrial CH4-based ectoine production.
Classification: Metano
ISSN: 0301-4797
Peer Review: NO
Sponsor: Ministerio de Economía, Industria y Competitividad (Project CTM2015-70442-R project and Red NOVEDAR)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. UIC71)
European Union through the FEDER Funding Program (PhD Grant contract Nº E-47-2014-0140696)
Publisher Version:
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:GTA - Artículos de revista
DEP48 - Artículos de revista

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