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Title: Tethering QK peptide to enhance angiogenesis in elastin-like recombinamer (ELR) hydrogels
Authors: Flora, Tatjana
González de Torre, Israel
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
Issue Date: 2019
Publisher: Springer Link
Description: Producción Científica
Citation: Journal of Materials Science: Materials in Medicine, 2019, vol. 30, n. 30. 12 p.
Abstract: The development of new capillary networks in engineered constructs is essential for their survival and their integration with the host tissue. It has recently been demonstrated that ELR-based hydrogels encoding different bioactivities are able to modulate their interaction with the host after injection or implantation, as indicated by an increase in cell adhesion and the ability to trigger vascularization processes. Accordingly, the aim of this study was to increase their angiogenic ability both in vitro and in vivo using a small VEGF mimetic peptide named QK, which was tethered chemically to ELR-based hydrogels containing cell-adhesion sequences in their backbone, such as REDV and RGD, as well as a proteolytic site (VGVAPG). In vitro studies were performed using a co-culture of endothelial and fibroblast cells encapsulated into the ELR-based hydrogels in order to determine cell proliferation after 21 days of culture, as well as the number of cell-cell interactions. It was found that although the presence of this peptide does not influence the morphological and rheological properties of these hydrogels, it has an effect on cell behaviour, inducing an increase in cell proliferation and the formation of endothelial cell clusters. In vivo studies demonstrate that the QK peptide enhances the formation of prominent functional capillaries at three weeks post-injection, as confirmed by H&E staining and CD31 immunohistochemistry. The newly formed functional microvasculature ensures perfusion and connection with surrounding tissues. These results show that ELR-QK hydrogels increase capillary network formation and are therefore attractive candidates for application in tissue regeneration, for example for the treatment of cardiovascular diseases such as myocardial infarction or ischemia.
Classification: Péptido QK
QK peptide
Hidrogeles de elastámero recombinante
Elastin-like recombinamer hydrogels
ISSN: 0957-4530
Peer Review: SI
Sponsor: European Commission (NMP-2014-646075, PITN-GA-2012-317306)
Ministerio de Economía, Industria y Competitividad (Projects PCIN-2015-010, MAT2015- 68901-R, MAT2016-78903-R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA015U16)
Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
Publisher Version:
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:BIOFORGE - Artículos de revista

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