|Title: ||Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers|
|Authors: ||Flora, Tatjana|
González de Torre, Israel
Quintanilla Sierra, Luis
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
|Issue Date: ||2018|
|Description: ||Producción Científica|
|Citation: ||European Polymer Journal, 2018, vol. 106. p. 19-29|
|Abstract: ||A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out.
After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof.
Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties.|
|Classification: ||Control espacial|
Recombinantes tipo elastina
|Peer Review: ||SI|
|Sponsor: ||Comisión Europea (NMP-2014-646075, MSCA-ITN-2014-642687)|
Ministerio de Economía, Industria y Competitividad (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R and MAT2016-79435-R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. Project VA015U16)
Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
|Publisher Version: ||https://www.sciencedirect.com/science/article/pii/S0014305717322061|
|Rights Owner: ||© 2019 Elsevier|
|Appears in Collections:||BIOFORGE - Artículos de revista|
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