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Title: Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers
Authors: Flora, Tatjana
González de Torre, Israel
Quintanilla Sierra, Luis
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
Issue Date: 2018
Publisher: Elsevier
Description: Producción Científica
Citation: European Polymer Journal, 2018, vol. 106. p. 19-29
Abstract: A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out. After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof. Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties.
Classification: Control espacial
Spatial control
Adhesión celular
Cell adhesion
Biofuncionalización superficial
Surface bio-functionalization
Ablación laser
Laser ablation
Recombinantes tipo elastina
Elastin-like recombinamers
ISSN: 0014-3057
Peer Review: SI
Sponsor: Comisión Europea (NMP-2014-646075, MSCA-ITN-2014-642687)
Ministerio de Economía, Industria y Competitividad (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R and MAT2016-79435-R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. Project VA015U16)
Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
Publisher Version:
Rights Owner: © 2019 Elsevier
Language: eng
Rights: info:eu-repo/semantics/embargoedAccess
Appears in Collections:BIOFORGE - Artículos de revista

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