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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/34908

    Título
    Cartilage Regeneration in Preannealed Silk Elastin-Like Co-Recombinamers Injectable Hydrogel Embedded with Mature Chondrocytes in an Ex Vivo Culture Platform
    Autor
    Cipriani, Filippo
    Krüger, Melanie
    González de Torre, IsraelAutoridad UVA Orcid
    Quintanilla Sierra, LuisAutoridad UVA
    Alonso Rodrigo, MatildeAutoridad UVA Orcid
    Kock, Linda
    Rodríguez Cabello, José CarlosAutoridad UVA Orcid
    Año del Documento
    2018
    Editorial
    American Chemical Society
    Descripción
    Producción Científica
    Documento Fuente
    Biomacromolecules, 2018, 19 (11), pp 4333–4347
    Abstract
    Tissue engineering for cartilage repair requires biomaterials that show rapid gelation and adequate mechanical properties. Although the use of hydrogel is the most promising biomaterial, it often lacks in rigidity and anchorage of cells when they are surrounded by synovial fluid while they are subjected to heavy loads. We developed and produced the Silk Elastin-Like co-Recombinamer (SELR), which contains both the physical interaction from elastin motifs and from silk motifs. In the first part of this work, we set up and optimized a preannealing treatment based on the evolution of silk motifs into β-sheet structures in order to fulfill the required mechanical properties of hydrogels for cartilage repair. The new preannealed SELRs (pA(EIS)2-(I5R)6) were characterized with the combination of several experimental techniques (CD, TEM, SEM, and rheology) to provide a deep insight into the material features. Finally, the regeneration properties of the pA(EIS)2-(I5R)6 hydrogel embedded with chondrocytes were evaluated. After 4 weeks of culturing in a standardized and representative ex vivo model, the biochemical and histological analysis revealed the production of glycosaminglycans and collagen. Moreover, the immunohistochemistry showed the absence of fibro-cartilage and the presence of hyaline cartilage. Hence, we conclude that the pA(EIS)2-(I5R)6 hydrogel presents improved mechanical properties while conserving the injectability, which leads to successful regeneration of hyaline cartilage in an ex vivo model.
    Palabras Clave
    Regeneración del cartílago
    Cartilage Regeneration
    Elastina
    Elastin
    ISSN
    1525-7797
    Revisión por pares
    SI
    DOI
    10.1021/acs.biomac.8b01211
    Patrocinador
    Ministerio de Economía, Industria y Competitividad (Proyects PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R,MAT2016-79435-R
    Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. Project VA015U16)
    European Union’sHorizon 2020 research and innovation programme under theMarie Sklodowska-Curie grant agreement No. 64268
    Patrocinador
    info:eu-repo/grantAgreement/EC/H2020/642687
    Version del Editor
    https://pubs.acs.org/doi/10.1021/acs.biomac.8b01211
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/34908
    Derechos
    openAccess
    Aparece en las colecciones
    • BIOFORGE - Artículos de revista [89]
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    Attribution-NonCommercial-NoDerivatives 4.0 InternationalLa licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 International

    Universidad de Valladolid

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