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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/66627

    Título
    Use of a highly specialized biocatalyst to produce lactate or biohydrogen and butyrate from agro-industrial resources in a dual-phase dark fermentation
    Autor
    García Depraect, OctavioAutoridad UVA Orcid
    León Becerril, Elizabeth
    Año del Documento
    2023
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    Fermentation, 2023, Vol. 9, Nº. 9, 787
    Résumé
    This study aimed at investigating the feasibility of using a highly specialized bacterial inoculum harboring lactic acid bacteria (LAB) and lactate-oxidizing, hydrogen-producing bacteria (LO-HPB) to produce either lactate or biohydrogen and butyrate from several agro-industrial resources via dual-phase dark fermentation. The feedstocks were fruit–vegetable waste, cheese whey, coffee wastewater, tequila vinasse, and maize processing wastewater, and were tested in both mono- and co-fermentation. The results obtained indicated that the biocatalyst used was able to perform a dual-phase lactate fermentation, producing high lactate (13.1–36.4 g/L), biohydrogen (0.2–7.5 NL H2/Lfeedstock, equivalent to 0.3–1.7 mol H2/mol hexose), and butyrate (3.3–13.9 g/L) with all the tested feedstocks. A series of self-fermentation tests were also performed with crude cheese whey and fruit–vegetable waste for comparison purposes. Compared to inoculum-aided fermentations, the self-fermentation exhibited a reduced bioconversion efficiency. Short-length 16S rRNA gene sequencing analysis showed that LO-HPB was the dominant microbial group (86.0%) in the biocatalyst, followed by acetic acid bacteria (5.8%) and LAB (5.7%). As expected, the molecular analysis also showed significant differences in the microbial community structure of the biocatalyst and those that evolved from self-fermentation. Besides lactate fermentation and oxidation, the biocatalyst also assisted the bi-phasic lactate fermentation via oxygen consumption, and apparently, via substrate hydrolysis. Overall, this study can lay the foundation for robust inoculum development, which is of special significance in the field of dark fermentation, and proposes an innovative bioprocess for agro-industrial valorization through a trade-off approach, tailoring the metabolic pathway to the target product(s).
    Materias (normalizadas)
    Hydrogen - Biotechnology
    Hydrogen as fuel
    Renewable energy resources
    Energías renovables
    Biotechnology
    Biotecnología
    Biorefinery
    Dark fermentation
    Organic acids
    Organic wastes
    Residuos orgánicos
    Materias Unesco
    2303.14 Hidrogeno
    5312.05 Energía
    2302.12 Fermentación
    2301 Química Analítica
    ISSN
    2311-5637
    Revisión por pares
    SI
    DOI
    10.3390/fermentation9090787
    Patrocinador
    Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT) - (Project-PN-2015-01-1024)
    Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación (AEI)/10.13039/501100011033 y Unión Europea NextGenerationEU/PRTR - (grant RYC2021-034559-I)
    Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - (grant CL-EI-2021-07 y UIC 315)
    Version del Editor
    https://www.mdpi.com/2311-5637/9/9/787
    Propietario de los Derechos
    © 2023 The authors
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/66627
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
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    • IPS - Artículos de revista [157]
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