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Título
Use of a highly specialized biocatalyst to produce lactate or biohydrogen and butyrate from agro-industrial resources in a dual-phase dark fermentation
Año del Documento
2023
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Fermentation, 2023, Vol. 9, Nº. 9, 787
Résumé
This study aimed at investigating the feasibility of using a highly specialized bacterial inoculum harboring lactic acid bacteria (LAB) and lactate-oxidizing, hydrogen-producing bacteria (LO-HPB) to produce either lactate or biohydrogen and butyrate from several agro-industrial resources via dual-phase dark fermentation. The feedstocks were fruit–vegetable waste, cheese whey, coffee wastewater, tequila vinasse, and maize processing wastewater, and were tested in both mono- and co-fermentation. The results obtained indicated that the biocatalyst used was able to perform a dual-phase lactate fermentation, producing high lactate (13.1–36.4 g/L), biohydrogen (0.2–7.5 NL H2/Lfeedstock, equivalent to 0.3–1.7 mol H2/mol hexose), and butyrate (3.3–13.9 g/L) with all the tested feedstocks. A series of self-fermentation tests were also performed with crude cheese whey and fruit–vegetable waste for comparison purposes. Compared to inoculum-aided fermentations, the self-fermentation exhibited a reduced bioconversion efficiency. Short-length 16S rRNA gene sequencing analysis showed that LO-HPB was the dominant microbial group (86.0%) in the biocatalyst, followed by acetic acid bacteria (5.8%) and LAB (5.7%). As expected, the molecular analysis also showed significant differences in the microbial community structure of the biocatalyst and those that evolved from self-fermentation. Besides lactate fermentation and oxidation, the biocatalyst also assisted the bi-phasic lactate fermentation via oxygen consumption, and apparently, via substrate hydrolysis. Overall, this study can lay the foundation for robust inoculum development, which is of special significance in the field of dark fermentation, and proposes an innovative bioprocess for agro-industrial valorization through a trade-off approach, tailoring the metabolic pathway to the target product(s).
Materias (normalizadas)
Hydrogen - Biotechnology
Hydrogen as fuel
Renewable energy resources
Energías renovables
Biotechnology
Biotecnología
Biorefinery
Dark fermentation
Organic acids
Organic wastes
Residuos orgánicos
Materias Unesco
2303.14 Hidrogeno
5312.05 Energía
2302.12 Fermentación
2301 Química Analítica
ISSN
2311-5637
Revisión por pares
SI
Patrocinador
Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT) - (Project-PN-2015-01-1024)
Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación (AEI)/10.13039/501100011033 y Unión Europea NextGenerationEU/PRTR - (grant RYC2021-034559-I)
Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - (grant CL-EI-2021-07 y UIC 315)
Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación (AEI)/10.13039/501100011033 y Unión Europea NextGenerationEU/PRTR - (grant RYC2021-034559-I)
Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - (grant CL-EI-2021-07 y UIC 315)
Version del Editor
Propietario de los Derechos
© 2023 The authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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