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Por favor, use este identificador para citar o enlazar este ítem: http://uvadoc.uva.es/handle/10324/21697
Título: Biocompatible ELR-Based Polyplexes Coated with MUC1 Specific Aptamers and Targeted for Breast Cancer Gene Therapy
Autor: Piña, María José
Girotti, Alessandra
Santos García, María Mercedes
Rodríguez Cabello, José Carlos
Arias Vallejo, Francisco Javier
Año del Documento: 2016
Editorial: American Chemical Society
Descripción: Producción Científica
Documento Fuente: Molecular Pharmaceutics, American Chemical, 2016, vol. 7; 13; (3) p. 795-808
Resumen: The search for new and biocompatible materials with high potential for improvement is a challenge in gene delivery applications. A cell type specific vector made of elastin- like recombinamer (ELR) and aptamers has been specifically designed for the intracellular delivery of therapeutic material for breast cancer therapy. A lysine-enriched ELR was constructed and complexed with plasmid DNA to give positively charged and stable polyplexes. Physical character- ization of these polyplexes showed a particle size of around 140 nm and a zeta potential of approximately +40 mV. The incorporation of MUC1-specific aptamers into the polyplexes resulted in a slight decrease in zeta potential but increased cell transfection specificity for MCF-7 breast cancer cells with respect to a MUC1-negative tumor line. After showing the transfection ability of this aptamer-ELR vector which is facilitated mainly by macropinocytosis uptake, we demonstrated its application for suicide gene therapy using a plasmid containing the gene of the toxin PAP-S. The strategy developed in this work about using ELR as polymeric vector and aptamers as supplier of specificity to deliver therapeutic material into MUC1-positive breast cancer cells shows promising potential and continues paving the way for ELRs in the biomedical field.
Materias (normalizadas): Cáncer-Terapias
ISSN: 1543-8384
Revisión por Pares: SI
DOI: 10.1021/acs.molpharmaceut.5b00712
Patrocinador: Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13)
Version del Editor: pubs.acs.org/molecularpharmaceutics
Idioma: eng
URI: http://uvadoc.uva.es/handle/10324/21697
Derechos: info:eu-repo/semantics/embargoedAccess
Aparece en las colecciones:DEP60 - Artículos de revista

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