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Title: Calcium Imaging in Neuron Cell Death
Authors: Calvo, María
Villalobos, Carlos
Nuñez Llorente, Lucía
Issue Date: 2015
Publisher: Springer Science+Business Media New York
Description: Producción Científica
Citation: Lossi, Laura and Merighi, Adalberto, editors. Neuronal Cell Death: Methods and Protocols, Springer, 2015, p. 73 – 85 (Methods in Molecular Biology; 1254)
Abstract: Intracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuron cell death. For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity. Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt) in neurons concurrently with apoptosis and neuron cell death is widely required. Procedures for culture and preparation of primary cultures of hippocampal rat neurons and fluorescence imaging of cytosolic Ca2+ concentration in Fura2/AM-loaded neurons are described. We also describe a method for apoptosis detection by immunofluorescence imaging. Finally, a simple method for concurrent measurements of [Ca2+]cyt and apoptosis in the same neurons is described.
Keywords: Calcium imaging
Neuron death
Hippocampal neurons
ISBN: 978-1-4939-2152-2
Sponsor: Ministerio de Economía y Competitividad (BFU2009-08967 and BFU2012-37146)
Junta de Castilla y León (BIO103/VA45/11, VA145U13, and BIO/VA33/13)
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Rights Owner: © Todos los derechos reservados © Springer Science+Business Media New York
Language: eng
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:DEP06 - Capítulos de monografías

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