|Title: ||Calcium Imaging in Neuron Cell Death|
|Authors: ||Calvo, María|
Nuñez Llorente, Lucía
|Issue Date: ||2015|
|Publisher: ||Springer Science+Business Media New York|
|Description: ||Producción Científica|
|Citation: ||Lossi, Laura and Merighi, Adalberto, editors. Neuronal Cell Death: Methods and Protocols, Springer, 2015, p. 73 – 85 (Methods in Molecular Biology; 1254)|
|Abstract: ||Intracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuron
cell death. For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity.
Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt) in neurons concurrently
with apoptosis and neuron cell death is widely required.
Procedures for culture and preparation of primary cultures of hippocampal rat neurons and fluorescence
imaging of cytosolic Ca2+ concentration in Fura2/AM-loaded neurons are described. We also
describe a method for apoptosis detection by immunofluorescence imaging. Finally, a simple method for
concurrent measurements of [Ca2+]cyt and apoptosis in the same neurons is described.|
|Keywords: ||Calcium imaging|
|Sponsor: ||Ministerio de Economía y Competitividad (BFU2009-08967 and BFU2012-37146)|
Junta de Castilla y León (BIO103/VA45/11, VA145U13, and BIO/VA33/13)
|Publisher Version: ||http://rd.springer.com/|
|Rights Owner: ||© Todos los derechos reservados
© Springer Science+Business Media New York|
|Appears in Collections:||DEP06 - Capítulos de monografías|
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