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Título
Screening enzymes that can depolymerize commercial biodegradable polymers: Heterologous expression of Fusarium solani cutinase in Escherichia coli
Autor
Año del Documento
2023
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Microorganisms, 2023, Vol. 11, Nº. 2, 328
Abstract
In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycling. However, the functional and systematic study of depolymerase enzymes with respect to the degradation of a series of plastic polymers in a single work has not been widely addressed at present. In this study, the ability of a set of enzymes (esterase, arylesterase and cutinase) to degrade commercial biodegradable polymers (PBS, PBAT, PHB, PHBH, PHBV, PCL, PLA and PLA/PCL) and the effect of pre-treatment methods on their degradation rate was assessed. The degradation products were identified and quantified by HPLC and LC-HRMS analysis. Out of the three enzymes, Fusarium solani cutinase (FsCut) showed the highest activity on grinded PBAT, PBS and PCL after 7 days of incubation. FsCut was engineered and heterologous expressed in Escherichia coli, which conferred the bacterium the capability of degrading solid discs of PBAT and to grow in PBS as the sole carbon source of the medium.
Materias (normalizadas)
Biodegradable plastics
Polymeric composites
Depolymerization
Polymers
Polymerization
Polimeros y polimerizacion
Plastics
Materias plásticas
Sustainable development
Desarrollo sostenible
Renewable natural resources
Recursos naturales renovables
Enzymes
Enzimas
Microbiology
Environment
Materias Unesco
2206.10 Polímeros
2302.09 Enzimología
2414 Microbiología
3308 Ingeniería y Tecnología del Medio Ambiente
ISSN
2076-2607
Revisión por pares
SI
Patrocinador
Société des Produits Nestlé S.A, Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - (grant CL-EI-2021-07 and UIC 315)
Version del Editor
Propietario de los Derechos
© 2023 The authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Collections
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